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ABSTRACT

J Appl Microbiol. 2003;95(1):196-201

Comparative evaluation of the MGIT and BACTEC culture systems for the recovery of Mycobacterium avium subsp. paratuberculosis from milk

Grant IR, Kirk RB, Hitchings E, Rowe MT
Department of Food Microbiology, Queen's University of Belfast, Belfast, UK

[received date and accepted date, if any]

AIMS: To compare the detection capabilities of the non-radiometric MGIT (Mycobacteria Growth Indicator Tubes) and radiometric BACTEC 460TB culture systems (Becton Dickinson, Cowley, Oxford, UK) for recovering Mycobacterium avium subsp. paratuberculosis from milk.

METHODS AND RESULTS: Ultra heat treated (UHT) milk samples spiked with different levels of M. paratuberculosis (10-107 cells ml-1) were inoculated into MGIT and BACTEC media (containing recommended supplements) with and without prior chemical decontamination of the milk samples with 0.75% (w/v) cetylpyridinium chloride for 5 h. Time for the detection of growth in days was recorded for each culture system, and a M. paratuberculosis count for each milk sample was calculated from BACTEC readings using a published formula. Correlation between MGIT and BACTEC detection times was 0.6983. Both culture systems were capable of detecting 10-100 M. paratuberculosis cells ml-1 in milk within 30-40 days when no decontamination treatment was applied, but only 102-103 cells ml-1 or greater when chemical decontamination was applied before culture.

CONCLUSIONS, SIGNIFICANCE AND IMPACT OF STUDY: The non-radiometric MGIT system could be substituted for the radiometric BACTEC system for the culture of M. paratuberculosis from milk without loss of detection sensitivity. Chemical decontamination before culture caused a significant reduction in numbers of viable M. paratuberculosis in all spiked milk samples resulting in decreased detection capability for both culture systems.


PARA's SUMMARY

THIS RESEARCH SHOWS A GOOD CORRELATION BETWEEN TWO DIFFERENT METHODS OF DETECTING THE PRESENCE OF MAP IN MILK.

DECONTAMINATION IS OFTEN USED TO PREVENT THE OVERGROWTH OF OTHER BACTERIA FROM AFFECTING THE MEASUREMENT OF THE RELATIVELY SLOW-GROWING MAP. THIS RESEARCH SHOWS HOW DECONTAMINATION DEGRADES THE SENSITIVITY OF TESTS FOR MAP.


Source: http://www.crohns.org/articles/2003_07_196-201_jam.htm   Contact PARA: http://www.crohns.org/contact.htm
Paratuberculosis Awareness & Research Association